What is eadie hofstee plot?

The Eadie-Hofstee plot is a graphical method used to determine enzyme kinetics parameters, particularly the Michaelis-Menten constant (Km) and the maximum reaction rate (Vmax). The plot is created by plotting the velocity (V) of an enzyme-catalyzed reaction against the ratio of velocity to substrate concentration (V/[S]). The slope of the line is equal to Vmax, and the x-intercept is equal to -1/Km. This method can be useful for determining the kinetic parameters of an enzyme when only limited concentrations of substrate and enzyme are available. However, it is often less accurate than other methods, such as nonlinear regression.